The time spent exploring the enclosures (with or without the stimulus mice) by the test subject is measured[YP1]. During the habituation session, the subject mouse usually explores zones 1 and 4 equally. During the sociability session, the subject mouse spends more time exploring the enclosure with ‘stranger 1’ mouse compared to the empty enclosure, indicating sociability. During the social novelty session, the subject mouse spends more time exploring with ‘stranger 2’ than ‘stranger 1’, indicating social novelty. By introducing a delay in social novelty testing after the initial sociability session, social memory can be assessed. The accuracy of our automated assessment has been validated manually.
Khroyan et al., 2012 -- add full reference and link here